Overall, there are several symptoms indicating that you may have COPD.
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Genes of interest (GOIs) up-regulated by cannabis oil extract at all three test dilutions (dose response experiment). HSAEpC were exposed to each of three dilutions of cannabis oil extract in ethanol for 24 h. Each fold change result is based on four cannabis oil extract treatment replicates and six control replicates. Legend: Black, 1:400 dilution; Grey, 1:800 dilution; Horizontal Stripes, 1:1600 dilution
Quantitative PCR results were analyzed by the delta cycle threshold (Ct) method, with the 0.25% ethanol treated cells as a control, using the Data Resources Center (www.Qiagen.com). Genes with Ct values > = 35 were excluded from this analysis, and genes with Ct values > 32 were considered low expression. Fold changes (2^(− Delta Ct)) were determined as the normalized gene expression (2^(− Delta Ct)) in the cannabis extract treated sample divided by the normalized gene expression (2^(− Delta Ct)) in the control sample (ethanol treated cells). Student’s t-test was used to determine p-values between control group (ethanol) and each experimental group (cannabis oil extract). Genes were regarded as up-regulated or down-regulated if their extract treatment fold changes were > 2.0 or < 0.5 relative to ethanol controls and were statistically significant (P < 0.05). The same analysis was performed to compare the results of the 0.25% ethanol treated cells with that of the saline control cells.
Another GOI up-regulated by cannabis oil extract was TSLP, which encodes thymic stromal lymphopoietin. TSLP protein is known to be expressed in lung epithelial cells, and is associated with the Th2 immune response, including the downstream production of Th2 cytokines and chemokines such as IL-4 and chemokine (C-C motif) ligand 22 (Fang et al. 2010; He and Geha 2010; Redhu and Gounni 2012). Also, TSLP protein, in conjunction with Th2 cytokine IL-13, was capable of increasing airway epithelial cell proliferation as well as cell repair after mechanical injury (Semlali et al. 2010).
RNA extraction and reverse transcription
HSAEpC were seeded in 6-well cell culture plates at a density of 200,000 cells per well. After overnight adhesion, cells were treated for 24 h with media containing cannabis oil extract (1:400, 1:800 and 1:1600 dilutions). Negative control cells were treated with an equivalent volume of ethanol or phosphate buffered saline (PBS) (1:400 dilution for a 0.25% final concentration). Each treatment dilution had four replicates, whereas control conditions (ethanol and PBS) had 6 replicates.
After the yield of RNA extraction was quantified using a Nanodrop (Thermo Fisher Scientific), complementary DNA (cDNA) was synthesized from 250 ng of RNA using the reverse transcription (RT2) First Strand kit (Qiagen, Valencia, CA) following the manufacturer’s instructions.
Cannabis sativa oil extract
Pathway analysis of the six genes of interest that were significantly down-regulated by the 1:400 and 1:800 dilutions of cannabis oil extract (CLCA1, CMA1, EPX, LTB4R, MAF and PMCH) did not reveal common biological functions. That said, two of the down-regulated GOIs, CLCA1 and CMA1, appear to be highly relevant to the treatment of COPD with respect to mucus overproduction or hypersecretion. CLCA1 encodes chloride channel accessory 1, whose expression or up-regulation has been related to mucus overproduction as well as airway hyperreactivity in COPD (Hauber et al. 2005; Iwashita et al. 2012; Sala-Rabanal et al. 2015; Wang et al. 2007). The feasibility of inhibiting mucin production genes as a viable approach to treating COPD has been postulated (Ha and Rogers 2016). Accordingly, the use of cannabis oil extract to inhibit the expression of mucin production genes such as CLCA1 could help in controlling airway mucus hypersecretion. CMA1 encodes mast cell chymase 1, a serine proteinase. Chymase can stimulate mucin secretion from lung epithelial cells, and is known to contribute to the mucus hypersecretion process in patients with COPD (He and Zheng 2004). Also, mast cell chymase and other serine proteases are known to promote inflammation through degradation of the extracellular matrix (de Garavilla et al. 2005). An inhibitor of these proteases had anti-inflammatory effects that could be related to the treatment of COPD. The down-regulation of CMA1 would therefore potentially decrease both mucus hypersecretion and inflammation.
Chronic obstructive pulmonary disease (COPD) is commonly associated with both a pro-inflammatory and a T-helper 1 (Th1) immune response. It was hypothesized that cannabis oil extract can alleviate COPD symptoms by eliciting an anti-inflammatory Th2 immune response. Accordingly, the effects of cannabis oil extract on the expression of 84 Th2 and related immune response genes in human small airways epithelial cells (HSAEpC) were investigated.
Another challenge with CBD dosage for COPD is that different products have different concentrations of CBD. A CBD oil tincture could range from 100mg to 1,500 mg of CBD and the concentration per milliliter depends on how many milliliters of liquid is in the bottle. For example, a 30ml (1 ounce) of 100mg CBD oil tincture contains about 3mg of CBD per ml. A 1000 mg concentration, however, contains over 30mg of cannabidiol per ml.
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CBD Oil for COPD Dosage
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The goal of therapy for people with COPD is to stop the progression of the disease and to improve lung function. A combination of oxygen therapy and pulmonary rehabilitation can go a long way in improving the symptoms of various lung diseases like COPD, but other symptoms can be a little more stubborn.
COPD Oil Treatment for COPD
There are no standards for CBD dosage because medical studies are still somewhat limited. Add to that the fact that CBD can help with a wide range of problems in different people, and you can see how it might be difficult to pinpoint a specific dosage recommendation.
The most commonly noted side effects of CBD  are: